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serum response elements  (Addgene inc)


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    Structured Review

    Addgene inc serum response elements
    Serum Response Elements, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sre+reporter+vector+559/SRE+reporter+vector_559+(Plasmid+%2382686)/us12570720-409-12-22
    Average 91 stars, based on 3 article reviews
    serum response elements - by Bioz Stars, 2026-09
    91/100 stars

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    Related Articles

    Activity Assay:

    Article Title: Engineered receptors for human cytomegalovirus that are orthogonal to normal human biology
    Article Snippet: Human PDGFRα isoform 1 (GenBank NM_006206.4) was cloned in to the NheI-XhoI sites of pCEP4 (Invitrogen) with a N-terminal HA leader (MKTIIALSYIFCLVFA), myc-tag, linker (GSPGGASG), and followed by the mature polypeptide (a.a. 24–1,089). .. For measuring signaling activity, a minimal promoter under the control of tandem serum response elements was subcloned from SRE reporter vector_559 (Addgene # 82686) [ ], a GFP-PEST fluorescent reporter [ ] was inserted at the AscI site, and the entire reporter cassette was inserted at the NruI site of pCEP4-myc-PDGFRα by Gibson assembly. ..

    Article Title: Engineered receptors for human cytomegalovirus and uses thereof
    Article Snippet: Plasmids Human PDGFRα isoform 1 (GenBank NM_006206.4; SEQ ID NO: 2) was cloned in to the NheI-XhoI sites of pCEP4 (Invitrogen) with a N-terminal HA leader (MKTIIALSYIFCLVFA; SEQ ID NO: 12), myc-tag, linker (GSPGGASG; SEQ ID NO: 13), and followed by the mature polypeptide (a.a. 24-1,089 of SEQ ID NO: 1). .. For measuring signaling activity, a minimal promoter under the control of tandem serum response elements was subcloned from SRE reporter vector 559 (Addgene #82686) (Reichhart et al., Angew Chem Int Ed Engl 55: 6339-6342, 2016), a GFP-PEST fluorescent reporter (Li et al., J Biol Chem 273: 34970-34975, 1998) was inserted at the AscI site, and the entire reporter cassette was inserted at the NruI site of pCEP4-myc-PDGFRα by Gibson assembly. ..

    Article Title: Engineered receptors for human cytomegalovirus that are orthogonal to normal human biology.
    Article Snippet: Human PDGFRα isoform 1 (GenBank NM_006206.4) was cloned in to the NheI-XhoI sites of pCEP4 (Invitrogen) with a N-terminal HA leader (MKTIIALSYIFCLVFA), myc-tag, linker (GSPGGASG), and followed by the mature polypeptide (a.a. 24–1,089). .. For measuring signaling activity, a minimal promoter under the control of tandem serum response elements was subcloned from SRE reporter vector_559 (Addgene # 82686) [64], a GFP-PEST fluorescent reporter [63] was inserted at the AscI site, and the entire reporter cassette was inserted at the NruI site of pCEP4-myc-PDGFRα by Gibson assembly. ..

    Control:

    Article Title: Engineered receptors for human cytomegalovirus that are orthogonal to normal human biology
    Article Snippet: Human PDGFRα isoform 1 (GenBank NM_006206.4) was cloned in to the NheI-XhoI sites of pCEP4 (Invitrogen) with a N-terminal HA leader (MKTIIALSYIFCLVFA), myc-tag, linker (GSPGGASG), and followed by the mature polypeptide (a.a. 24–1,089). .. For measuring signaling activity, a minimal promoter under the control of tandem serum response elements was subcloned from SRE reporter vector_559 (Addgene # 82686) [ ], a GFP-PEST fluorescent reporter [ ] was inserted at the AscI site, and the entire reporter cassette was inserted at the NruI site of pCEP4-myc-PDGFRα by Gibson assembly. ..

    Article Title: Engineered receptors for human cytomegalovirus and uses thereof
    Article Snippet: Plasmids Human PDGFRα isoform 1 (GenBank NM_006206.4; SEQ ID NO: 2) was cloned in to the NheI-XhoI sites of pCEP4 (Invitrogen) with a N-terminal HA leader (MKTIIALSYIFCLVFA; SEQ ID NO: 12), myc-tag, linker (GSPGGASG; SEQ ID NO: 13), and followed by the mature polypeptide (a.a. 24-1,089 of SEQ ID NO: 1). .. For measuring signaling activity, a minimal promoter under the control of tandem serum response elements was subcloned from SRE reporter vector 559 (Addgene #82686) (Reichhart et al., Angew Chem Int Ed Engl 55: 6339-6342, 2016), a GFP-PEST fluorescent reporter (Li et al., J Biol Chem 273: 34970-34975, 1998) was inserted at the AscI site, and the entire reporter cassette was inserted at the NruI site of pCEP4-myc-PDGFRα by Gibson assembly. ..

    Article Title: Engineered receptors for human cytomegalovirus that are orthogonal to normal human biology.
    Article Snippet: Human PDGFRα isoform 1 (GenBank NM_006206.4) was cloned in to the NheI-XhoI sites of pCEP4 (Invitrogen) with a N-terminal HA leader (MKTIIALSYIFCLVFA), myc-tag, linker (GSPGGASG), and followed by the mature polypeptide (a.a. 24–1,089). .. For measuring signaling activity, a minimal promoter under the control of tandem serum response elements was subcloned from SRE reporter vector_559 (Addgene # 82686) [64], a GFP-PEST fluorescent reporter [63] was inserted at the AscI site, and the entire reporter cassette was inserted at the NruI site of pCEP4-myc-PDGFRα by Gibson assembly. ..

    Plasmid Preparation:

    Article Title: Engineered receptors for human cytomegalovirus and uses thereof
    Article Snippet: Plasmids Human PDGFRα isoform 1 (GenBank NM_006206.4; SEQ ID NO: 2) was cloned in to the NheI-XhoI sites of pCEP4 (Invitrogen) with a N-terminal HA leader (MKTIIALSYIFCLVFA; SEQ ID NO: 12), myc-tag, linker (GSPGGASG; SEQ ID NO: 13), and followed by the mature polypeptide (a.a. 24-1,089 of SEQ ID NO: 1). .. For measuring signaling activity, a minimal promoter under the control of tandem serum response elements was subcloned from SRE reporter vector 559 (Addgene #82686) (Reichhart et al., Angew Chem Int Ed Engl 55: 6339-6342, 2016), a GFP-PEST fluorescent reporter (Li et al., J Biol Chem 273: 34970-34975, 1998) was inserted at the AscI site, and the entire reporter cassette was inserted at the NruI site of pCEP4-myc-PDGFRα by Gibson assembly. ..



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    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: Nucleoporin-93 reveals a common feature of aggressive breast cancers: robust nucleocytoplasmic transport of transcription factors

    doi: 10.1016/j.celrep.2022.110418

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: SRE luciferase , Addgene , Cat# 82686.

    Techniques: Control, Recombinant, Isolation, SYBR Green Assay, Reverse Transcription, Reporter Assay, Enzyme-linked Immunosorbent Assay, Activation Assay, Luciferase, RNA Sequencing, Software, Plasmid Preparation